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MB49 Murine Bladder Carcinoma Cell Line
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Image Search Results
Journal: Journal of Clinical Medicine
Article Title: Nuclear Factor-κB Overexpression is Correlated with Poor Outcomes after Multimodality Bladder-Preserving Therapy in Patients with Muscle-Invasive Bladder Cancer
doi: 10.3390/jcm8111954
Figure Lengend Snippet: NFκB signaling was up-regulated after irradiation (RT) and inhibited with the treatment of an NFκB inhibitor, SC75741, mitigating the proliferative capability of bladder cancer cells. ( A ) Images and quantification by densitometry and ImageJ of Western blots for NFκB transcription factor proteins (p65 and p50) (n = 3) (n = 3) and ( B ) colony formation assays of MB49 and T24 cancer cells after pre-treatment with or without SC75741 (5 μM) and/or irradiation (5 Gy) (n = 4). * p < 0.05.
Article Snippet: Human bladder urothelial carcinoma cell line, T24, and murine bladder tumor cell line,
Techniques: Irradiation, Western Blot
Journal: Journal of Clinical Medicine
Article Title: Nuclear Factor-κB Overexpression is Correlated with Poor Outcomes after Multimodality Bladder-Preserving Therapy in Patients with Muscle-Invasive Bladder Cancer
doi: 10.3390/jcm8111954
Figure Lengend Snippet: Blockade of NFκB signaling by RNA interference inhibited radiation (RT)-induced nuclear translocation of phosphor-p65 and the invasiveness capability of murine bladder cancer cells. ( A ) Immunofluorescence staining of phospho-p65 at 0, 1, and 3 h after RT (5 Gy) of MB49 vector-control (Ctrl) and MB49 NFκB knock-down (KD) cells. ( B ) MB49 control (Ctrl) and MB49 NFκB KD cells were seeded in Matrigel-coated inserts of Boyden chambers, treated without and with RT (5 Gy). After 24 h, the invading cells were viewed microscopically (high-power field, 200 ×, left panel). Invading cells were counted. * p < 0.05, Ctrl sham group vs. Ctrl RT group.
Article Snippet: Human bladder urothelial carcinoma cell line, T24, and murine bladder tumor cell line,
Techniques: Translocation Assay, Immunofluorescence, Staining, Plasmid Preparation, Control, Knockdown